Introduction

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The Shibata Laboratory

Our laboratory aims to elucidate the molecular mechanism underlying DNA double-strand break (DSB) repair, which is critical for maintaining genome integrity. Defects in DSB repair lead to severe mutations, including deletions and chromosomal translocations, which cause cell death. Introducing such severe mutations is important for killing cancer cells via radio/chemotherapy. In addition, DSB repair and signaling is involved in many biological events such as tumorigenesis, immunity, protection against virus, and biological development.
  We are currently investigating the interplay between non-homologous end joining (NHEJ) and homologous recombination (HR), which are major DSB repair pathways. We have recently attempted to visualize a repair event at a single DSB using super-resolution microscope 3D structured-illumination microscopy (SIM). We have also conducted several ongoing projects with radiation oncologists on combined radio- and immunotherapy.

News!!

●2019.10.7
[NEW!! ]
Review article written by Dr. Atsushi Shibata and Dr. Hiro Sato  has been published in Cancer Science.
「Regulation of programmed death‐ligand 1 expression in response to DNA damage in cancer cells: Implications for precision medicine」
https://onlinelibrary.wiley.com/doi/full/10.1111/cas.14197
(Facebook Here

●2019.9.2
Dr. Atsushi Shibata presented our work at ICRR 2019 (Manchester,UK).
Title1 「Regulation of DNA damage signalling-dependent PD-L1 expression in cancer cells」
Title2 「Regulation of transcription-associated end-joining mediated by MRE11, BRCA1, Polθ and LIG1/3」
(Facebook Here

●2019.6.19
Member is updated.(Facebook Here

●2019.4.18
Iino-san and Takashima-san jointed Shibata lab as a secretary.(Facebook Here

●2019.4.12
There was a farewell party for Bobby-san last month.
Congratulation on your PhD! We appreciate your hard work in Japan.(Facebook Here


●2019.4.5
An interview of our recent study about transcription-associated DSB repair has been published.(Facebook Here
https://www.ingentaconnect.com/content/sil/impact/2019/00002019/00000003/art00019?crawler=true&mimetype=application/pdf  

●2019.4.1
Shibata lab moved to GIAR in Gunma University. (Facebook Here
https://www.giar.gunma-u.ac.jp/en/ 

●2019.3.24
Review article written by Dr. Atsushi Shibata and Dr. Hiro Sato  has been published in Frontiers in Oncology.
「Novel approaches to improve the efficacy of immuno-radiotherapy」(Facebook Here
https://www.frontiersin.org/…/10.3…/fonc.2019.00156/abstract

●2019.3.18
Sangeeta received Dean’s Award for excellent presentation in the quality examination of PhD course. Congratulations!(Facebook Here

●2019.2.18
Mayang’s paper has been published  in Oncogene. Congratulations!!
「Base excision repair regulates PD-L1 expression in cancer cells」
https://www.ncbi.nlm.nih.gov/pubmed/30755733
*Corresponding author: Atsushi Shibata(Facebook Here

●2019.1.28
Dr. Atsushi Shibata presented our work at Massachusetts General Hospital: MGH Cancer Center. (Boston, USA)
Title 「Regulation of transcription-associated DNA double strand break repair pathway choice 」(Facebook Here

●2019.1.27
Poster presentation at Keystone Symposia : DNA Replication and Genome Instability: From Mechanism to Disease.
「RAP80 suppresses Artemis-dependent deleterious rejoining during transcription-associated DNA double-strand break repair in the G1 phase」(Facebook Here

●2019.1.17
Mayang’s paper demonstrating the regulation of PD-L1 following oxidative DNA damage is accepted in Oncogene. Congratulations!!
*Corresponding author: Atsushi Shibata(Facebook Here

●2019.1.11
Review article written by Dr. Atsushi Shibata and Prof. Penny Jeggo  has been published in International Journal of Radiation Biology.
「A historical reflection on our understanding of radiation-induced DNA double strand break repair in somatic mammalian cells; interfacing the past with the present」
https://www.tandfonline.com/doi/abs/10.1080/09553002.2018.1564083?journalCode=irab20
(Facebook Here

●2018.11.27
Yoshihiko’s paper has been published in Journal of Radiation Research.
「Clustered DNA double-strand break formation and the repair pathway following heavy-ion irradiation」

https://academic.oup.com/jrr/advance-article/doi/10.1093/jrr/rry096/5196526
*Corresponding author: Atsushi Shibata(Facebook Here

●2018.11.27
Review article written by Dr. Atsushi Shibata and Prof. Penny Jeggo  has been published in Targeting the DNA Damage Response for Anti-Cancer Therapy「ATM: Its Recruitment, Activation, Signalling and Contribution to Tumour Suppression」
https://link.springer.com/chapter/10.1007/978-3-319-75836-7_6
(Facebook Here


●2018.11.15
Dr. Atsushi Shibata and Yoshihiko Hagiwara presented their work at The 34th Radiation Biology Center International Symposium / The 2nd International Symposium on Radiation Therapeutics and Biology”Molecular Targets and Precision Cancer Medicine: From basic research toward translation”(Facebook Here

●2018.10.30
Our collaborator  Dr. Sangeeta (Prof. Nakano lab)  received the ‘Best Presentation Award’ at The 6th International Symposium of Gunma University ( Program for Cultivating Global Leaders in Heavy Ion Therapeutics and Engineering). Congratulations!!(Facebook Here

●2018.10.2
Dr. Yasuhara’s (Taka’s) paper is highlighted in Nature Reviews – Molecular Cell Biology and Cancer Discovery.(Facebook Here
Transcriptionally tailored break repair
Nature Reviews – Molecular Cell Biology
https://www.nature.com/articles/s41580-018-0069-z

RAD52 Promotes Transcription-Associated Homologous Recombination Repair
Cancer Discovery
http://cancerdiscovery.aacrjournals.org/content/early/2018/09/27/2159-8290.CD-RW2018-165


●2018.9.21
Our collaborator  Dr. Yasuhara’s (Taka’s) paper  has been published in Cell.  Congratulations!!(Facebook Here
「Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair」
https://www.cell.com/cell/fulltext/S0092-8674(18)31117-6
*Co-corresponding author: Atsushi Shibata

●2018.9.12
Dr. Atsushi Shibata, Yoshihiko Hagiwara, Dr. 
Hiro Sato presented their work at The 3rd FARO Meeting 2018.(Facebook Here

●2018.9.3
Our collaborator  Prof. Nakano lab  has been published in Oncotarget .  Congratulations!!(Facebook Here
「 Mutational analysis of uterine cervical cancer that survived multiple rounds of radiotherapy 」
http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=25982

●2018.8.27
Taka’s paper regarding transcription associated DSB repair in G2 has been accepted for publication in Cell. Congratulations!! (Facebook Here
*Corresponding author: Atsushi Shibata


●2018.7.30
Poster presentation at Gordon Research Conference Genomic Instability,Chromosome Replication, Repair and Architecture(Facebook Here
「RAP80 regulates transcription-associated DNA end resection in G1」


●2018.6.8
Yoshihiko’s paper has been published in Human Immunology.(Facebook Here
「Analysis of programmed death-ligand 1 expression in primary normal human dermal fibroblasts after DNA damage」  
*Corresponding author: Atsushi Shibata
https://www.sciencedirect.com/science/article/pii/S0198885918301587

●2018.6.1
Yoshihiko’s paper has been accepted for publication in Human Immunology.  Congratulations!! (Facebook Here
*Corresponding author: Atsushi Shibata

●2018.5.29
Dr. Atsushi Shibata presented our work at Sussex University  Genome Damage Stability Centre.(Facebook Here
「Regulation of transcription-associated DNA double strand break repair pathway choice」

●2018.5.28
Poster presentation at Abcam Conference “Mechanisms of Recombination”
「RAP80 regulates transcription-associated DNA end resection in G1」(Facebook Here

●2018.3.21
Poster presentation at Radiation Break-through: from DNA damage responses to precision cancer therapy (Facebook Here
「DNA double-strand break repair pathway regulates PD-L1 expression in cancer cells」

●2018.1.12
Yoshihiko’s paper has been published in Oncotarget. (Facebook Here
「3D-structured illumination microscopy reveals clustered DNA double-strand break formation in widespread γH2AX foci after high LET heavy-ion particle radiation」
*Corresponding author: Atsushi Shibata
http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=22679&pubmed-linkout=1

●2017.11.27
Our collaborator Dr. Sato’s paper has been published in Nature Communications.
(Facebook Here
「DNA double-strand break repair pathway regulates PD-L1 expression in cancer cells」
*Corresponding author: Atsushi Shibata
http://www.nature.com/articles/s41467-017-01883-9

●2017.11.13

Yoshihiko’s paper has been accepted for publication in Oncotarget. Congratulations!! (Facebook Here

●2017.11.2
Our collaborator  Prof. Nakano lab  has been published in Journal of Visualized Experiments(JoVE).  Congratulations!!(Facebook Here
「One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy」
https://www.jove.com/video/56338/one-step-protocol-for-evaluation-mode-radiation-induced-clonogenic

●2017.10.30
Prof. Penny Jeggo visited Gunma university from Oct 21 to Oct 24.
We went to Gunma Museum of Natural History with our lab member.(Facebook Here

 ●2017.10.27
Our collaborator Dr. Sakasai’s paper has been published in Scientific Reports.
Congratulations!! (Facebook Here
「Aquarius is required for proper CtIP expression and homologous recombination repair」
http://www.nature.com/articles/s41598-017-13695-4

●2017.10.24
Our collaborator Dr. Sato’s paper from Prof. Nakano lab has been accepted for publication in Nature Communications.
Congratulations!! (Facebook Here

●2017.7.5
Our collaborator Nakako’ paper has been published in Oncology Reports.
Congratulations!! (Facebook Here
「Inhibition of the HDAC/Suv39/G9a pathway restores the expression of DNA damage-dependent major histocompatibility complex class I-related chain A and B in cancer cells」
*Corresponding author: Atsushi Shibata
https://www.spandidos-publications.com/10.3892/or.2017.5773

●2017.7.2
Dr. Atsushi Shibata participated in the 2nd annual meeting of the Japanese society for genome editing. Also, Dr. Atsushi Shibata joined the satellite meeting as an Expert of Endogenous DNA repair.(Facebook Here

●2017.6.16
Our collaborator Dr. Oike’s paper has been accepted for publication in Journal of Visualized Experiments(JoVE).
Congratulations!! (Facebook Here


●2017.5.25
Dr. Atsushi Shibata presented our work at 6th US-Japan DNA Repair Meeting. (Berkeley, USA)
Title「Spatiotemporal regulation of DNA double strand break repair pathway choice in G2-phase」(Facebook Here

●2017.4.6
Our collaborator Nakako’s paper has been accepted for publication in Oncology Reports.
Congratulations!! (Facebook Here

●2017.4.4
We had a cherry blossom viewing party at Shikishima park.(Facebook Here

●2017.2.17
Hiro Sato received the Excellent Prese!!
「Molecular mechanism of PD-L1 expression induced by X-ray irradiation」(Facebook Here
http://isrnb.org/conference/the-7th-isrn-coference/


●2017.1.30
Dr. Atsushi Shibata presented our work about PD-1 therapy in International Symposium on Immune Diversity and Cancer Therapy Kobe 2017.
Title「DNA repair pathway regulates PD-L1 expression in cancer cells」 (Facebook Here


●2017.1.30
The study from Markus Lobrich’s, Penny Jeggo’s and our lab has been published in Molecular Cell. Congratulations!!
「DNA Double-Strand Break Resection Occurs during Non-homologous End Joining in G1 but Is Distinct from Resection during Homologous Recombination」
http://www.cell.com/molecular-cell/fulltext/S1097-2765(16)30854-1


●2017.1.18
Our collaborator Dr. Kobayashi’s paper from Prof. Nakano lab has been published in Scientific Reports.Congratulations!!
「Mitotic catastrophe is a putative mechanism underlying the weak correlation between sensitivity to carbon ions and cisplatin」
https://www.ncbi.nlm.nih.gov/pubmed/28091564

●2017.1.10
ntation Award at the 7th International Society of Radiation Neurobiology(ISRN). Congratulations
 Mayu’s paper is published in Cell Reports
「BRCA1 Directs the Repair Pathway to Homologous Recombination by Promoting 53BP1 Dephosphorylation」 (Facebook Here

http://www.cell.com/cell-reports/fulltext/S2211-1247(16)31747-8


●2016.11.22

Mayu Isono received the Best Poster Award (concurrent division) at The 10th 3R Symposium (Facebook Here

●2016.11.22
Atsuko Niimi, Mayu Isono, Yoshihiko Hagiwara presented their work at poster presentation in The 10th 3R Symposium.
Niimi Atsuko「Analysis of DNA synthesis during homologous recombination in G2 cells after ionizing irradiation」
Mayu Isono「BRCA1 directs the repair pathway to homologous recombination by promoting 53BP1 dephosphorylation」
Yoshihiko Hagiwara 「The effect of Linear Energy Transfer in DNA double strand break repair pathway choice in G2 cells」

●2016.5.23
Poster presentation at Abcam Conference “Mechanisms of Recombination”.
「BRCA1 directs the repair pathway to homologous recombination by promoting 53BP1 dephosphorylation」
(Facebook is updated, https://www.facebook.com/pages/Atsushi-LAB/374216429402940

●2016.4.27

Atsuko’s paper has been accepted for publication in the Genes, Chromosomes and Cancer.

「Identification of DNA Double Strand Breaks at Chromosome Boundaries Along the Track of Particle Irradiation」
*Corresponding author: Atsushi Shibata

http://www.ncbi.nlm.nih.gov/pubmed/27113385
(Facebook is updated, https://www.facebook.com/pages/Atsushi-LAB/374216429402940


●2016.4.1

Dr. Mayu Isono joined our Lab.


●2016.4.1

Lab picnic

(Facebook is updated, https://www.facebook.com/pages/Atsushi-LAB/374216429402940


●2016.3.28

Poster presentation at 14th International Workshop on Radiation Damage to DNA.

「Analysis of cluster and DNA double strand break after heavy ion irradiation using high resolution microscopy」

(Facebook is updated, https://www.facebook.com/pages/Atsushi-LAB/374216429402940


●2016.3.1

The work with Radiation Oncology lab has been accepted for publication in the Scientific Reports.

「Visualization of complex DNA double-strand breaks in a tumor treated with carbon ion radiotherapy」
*Corresponding author: Atsushi Shibata

http://www.ncbi.nlm.nih.gov/pubmed/26925533


●2015.7.22

Facebook is updated. https://www.facebook.com/pages/Atsushi-LAB/374216429402940
(but only in Japanese)


●2015.4.6

Facebook is updated. https://www.facebook.com/pages/Atsushi-LAB/374216429402940
( Lab party at Shikishima park.)

2015.4.1
Omi-san jointed Shibata lab.